Mouse Anti-VEGFA Recombinant Antibody (clone mAb G6-31) (CAT#: HPAB-0330CQ)
This product is a recombinant mouse antibody that recognizes VEGFA. G6-31 recognises a VEGF epitope which is conserved between human and mouse VEGF and overlaps with the receptor binding surface.
Specific Inquiry
SDS-PAGE
Figure 1 Mouse Anti-VEGFA Recombinant Antibody (clone mAb G6-31) (HPAB-0330CQ) in SDS-PAGE
SDS-PAGE analysis of HPAB-0330CQ in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Mouse Anti-VEGFA Recombinant Antibody (clone mAb G6-31) (HPAB-0330CQ) in SEC-HPLC
The purity of HPAB-0330CQ was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-VEGFA Recombinant Antibody (clone mAb G6-31) (HPAB-0330CQ) in WB
Western blot analysis of HPAB-0330CQ was performed by loading
0.4 µg (lane 1), 0.8 µg (lane 2) and 1 µg (lane 3) VEGFA antigen onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-VEGFA Antibody (HPAB-0330CQ) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with goat anti-mouse IgG HRP secondary antibody at a dilution of 1:5,000 for one hour. Chemiluminescent was detected.
ELISA
Figure 4 Mouse Anti-VEGFA Recombinant Antibody (clone mAb G6-31) (HPAB-0330CQ) in ELISA
ELISA analysis of HPAB-0330CQ (Lot#CB2403J02L) was performed by coating with VEGFA antigen. Then blocked with BSA, and incubated with Anti-VEGFA Antibody (HPAB-0330CQ-L) at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 5 Mouse Anti-VEGFA Recombinant Antibody (clone mAb G6-31) (HPAB-0330CQ) in DB
Dot Blot analysis of HPAB-0330CQ was performed by coating with VEGFA antigen.
The secondary antibody: HRP-anti-Mouse IgG
Specifications
- Immunogen
- mVEGF
- Host Species
- Mouse
- Derivation
- Chimeric (mouse/human)
- Type
- Chimeric (mouse/human) IgG
- Specificity
- Human VEGFA
- Species Reactivity
- Human, Mouse
- Clone
- mAb G6-31
- Applications
- ELISA, IF, Block, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Blocking and Functional Assay. Please see the Published Data for further information.
Target
- Alternative Names
- Vascular Endothelial Growth Factor A; Vascular Permeability Factor; VEGF; VPF; Vascular Endothelial Growth Factor A121; Vascular Endothelial Growth Factor A165; Vascular Endothelial Growth Factor; VEGF-A; MVCD1;
- Gene ID
- 22339
- UniProt ID
- Q00731
Related Resources
Shen, Jingjing, et al. "Eyedrop-based macromolecular ophthalmic drug delivery for ocular fundus disease treatment." Science advances 9.4 (2023): eabq3104. https://doi.org/10.1126/sciadv.abq3104
This study focuses on developing an innovative delivery system for macromolecular ophthalmic drugs to treat ocular fundus diseases using eyedrop formulations. Traditional treatments like intravitreal injections present risks such as infections and retinal detachment. This research introduces a penetrating carrier based on fluorocarbon-modified chitosan (FCS), which can self-assemble with proteins to form nanocomplexes. These nanocomplexes effectively traverse ocular barriers to reach the posterior eye segments, demonstrating superior therapeutic responses in mouse models of choroidal melanoma and choroidal neovascularization compared to conventional methods. The FCS/anti-PDL1 and FCS/anti-VEGFA eyedrops induced stronger antitumor immune responses and inhibited vascular proliferation, respectively, showcasing the potential for at-home treatment of various eye diseases.
In this study, Creative Biolabs provided crucial therapeutic antibodies that were integral to the research. Specifically, they supplied anti-VEGFA (Cat#: HPAB-0330CQ), which was used in the formulation of FCS/anti-VEGFA nanocomplexes for treating choroidal neovascularization. This antibody played a pivotal role in achieving comparable therapeutic effects to intravitreal injections, thus validating the efficacy of the new delivery system. The contributions of Creative Biolabs significantly advanced the study, highlighting the potential for noninvasive, effective treatment options for ocular diseases.
More Infomation
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H73 | Anti-Human VEGFA Recombinant Antibody (TAB-H73) | WB, FC, IP, ELISA, Neut, FuncS, IF | VH - C-kappa - CH2 - CH3 _ V-lambda - CH1 _ H-GAMMA-1 _ L-kappa |
| TAB-307CQ | Human Anti-VEGFA Recombinant Antibody (TAB-307CQ) | Inhib | Human IgG |
| TAB-307CQ-S(P) | Human Anti-VEGFA Recombinant Antibody; scFv Fragment (TAB-307CQ-S(P)) | ELISA, Neut | Human scFv |
| TAB-307CQ-F(E) | Human Anti-VEGFA Recombinant Antibody; Fab Fragment (TAB-307CQ-F(E)) | ELISA, Neut | Humanized Fab |
| TAB-009ML | Anti-Human VEGFA Recombinant Antibody scFv Fragment (TAB-009ML) | ELISA, IHC, FC, IP, IF, Inhib | scFv, κ |
Customer Reviews and Q&As
Customer Review
Q&As
Michael White
Excellent for VEGFA Research
Excellent for VEGFA Research
The Anti-VEGFA Recombinant Antibody has been excellent for our VEGFA studies. Its specificity and sensitivity are outstanding, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
20-Aug-23
Laura Scott
Effective and Dependable
Effective and Dependable
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for VEGFA is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
04-Nov-22
Brian Young
Consistent High-Quality Results
Consistent High-Quality Results
This antibody has proven to be consistent and dependable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for VEGFA have been particularly beneficial.
28-Jun-21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0330CQ, RRID: AB_3111386)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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